Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.

نویسندگان

  • Qiang Wang
  • Leiyun Weng
  • Hongbing Jiang
  • Shijian Zhang
  • Tetsuya Toyoda
چکیده

Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

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عنوان ژورنال:
  • Analytical biochemistry

دوره 433 2  شماره 

صفحات  -

تاریخ انتشار 2013